SureFood® ALLERGEN Fish

Intended use: The a real-time PCR for the direct, qualitative and / or quantitative detection of specific fish DNA sequences according to directive (EC) 1169/2011. The group of fishes contains about 30000 species. Due to the large number of different species a practical verification of all is impossible. More detailed information is available in the Validation Report. For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg fish / kg food sample and a detection system including a standard series is required.
Specifications:

- Test format: 100 reactions
- Sample preparation: For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
- LOD (Detection limit): The PCR has a limit of detection of = 1 mg / kg using SureFood® PREP Advanced, protocol 1.
- LOQ (Limit of quantification): 4 mg / kg using SureFood® PREP Advanced, protocol 1
- Cross reactivity:

Cross reactivity was observed with DNA extracts from muscovy duck (Cairina moschata).
- Accessories: SureFood® PREP Advanced SureFood® QUANTARD Allergen 40 SureFast® Mag PREP Food
- Related products: RIDA®CYCLERTANBead Maelstrom™ 4800SureFood® ALLERGEN CrustaceansSureFood® ALLERGEN Molluscs
General Information:
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96 Dx, R-Biopharm RIDACYCLER and Agilent AriaDx.