Minute TM High Efficiency Protein Precipitation kit WA-006

Minute TM High Efficiency protein precipitation kit

Manual/protocol

MSDS sheet

Protein precipitation is an obvious choice for concentrating proteins and removing interfering substances in protein samples such as salts, lipid and other components that may interfere with downstream applications. One of the most commonly used methods is trichloroacetic acid (TCA)/acetone precipitation. This is a relatively simple and effective method. Proteins precipitated by TAC/acetone method is usually denatured and in many cases the solubility of protein is reduced. A major disadvantage of traditional TCA/acetone precipitation method is its low efficiency for the samples of low protein concentration. The higher protein concentration sample precipitate much more effectively than the lower protein concentration samples. In order to overcome the shortcomings, we have developed this high efficiency protein precipitation kit, which is a modification of traditional TCA method. It is simple, rapid and highly effective. Low protein concentration samples can be effectively precipitated and concentrated in about 25-30 min. The precipitated protein can be re-dissolved readily in detergent containing buffers.

Kit includes: Items

Quantity

Protein Precipitation Solution

30 ml

Washing Solution

30 ml

a7951